Researchers in the United States have revealed that MRSA repeats mutations. sarZ Genes leading to increased severity of bloodstream infections in mouse models.
Researchers at Mount Sinai Hospital in the United States, in collaboration with researchers at New York University in the United States, have shed light on the mechanisms behind the severity of methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infection.
Research published in Cell hosts and microorganismsrevealed that MRSA is recurrently mutated. sarZ gene, a transcriptional regulator involved in regulating virulence gene expression, leading to increased severity of bloodstream infection in mouse models.
Widespread community-associated MRSA USA300 strains have recently become a leading cause of hospital-associated disease. bloodstream infection (BSI).
The researchers took advantage of USA300’s recent introduction into hospitals and its limited genetic variation to find mutations that contribute to its success in new settings. Researchers found that USA300 infection exhibited altered virulence control.
Using comparative genomics, they discovered the genes involved in this phenotype and found recurrent independent mutations in the transcriptional regulator sarZ. These mutations increased the virulence of he USA300 BSI isolates in a mouse model of BSI. The sarZ mutation resulted in increased expression and production of the surface protein ClfB, which was shown to be important for the pathogenesis of USA300 BSI isolates.
MRSA is endemic throughout the United States and causes a variety of diseases, including invasive bloodstream infections associated with high mortality. The goal of this study was to identify potential mechanisms by which MRSA adapted to the invasive environment of infection.
“Our study results provide a better understanding of the factors that contribute to MRSA virulence and may ultimately help uncover new therapeutic approaches,” said the study’s corresponding author.
“Continuous evolution of MRSA has changed the way virulence is regulated in bloodstream infections. It suggests that fitness can be optimized. toxicity”
This study focuses on the USA300 strain of MRSA, and future studies will investigate additional strains and indications in methicillin-susceptible Staphylococcus aureus infections (MSSA).